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May 10, 2020 21:25
4 yrs ago
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English term
sensitivity and specificity extended beyond assay
English to Russian
Medical
Medical: Instruments
The automated RNAScope assay, used to generate this data is similar to other ISH assays and requires overnight processing...
The VENTANA PD-L1 (SP263) rabbit mAb assay was used in this study. IHC assays such as Dako PD-L1 IHC 22C3 and 28–8 assays have been shown to demonstrate good concordance with each other; the sensitivity and specificity of RNAScope could therefore be inferred to extended beyond the VENTANA PD-L1 (SP263) rabbit mAb assay to the Dako PD-L1 IHC 22C3 and 28–8 assays.
The VENTANA PD-L1 (SP263) rabbit mAb assay was used in this study. IHC assays such as Dako PD-L1 IHC 22C3 and 28–8 assays have been shown to demonstrate good concordance with each other; the sensitivity and specificity of RNAScope could therefore be inferred to extended beyond the VENTANA PD-L1 (SP263) rabbit mAb assay to the Dako PD-L1 IHC 22C3 and 28–8 assays.
Discussion
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по чувствительности и специфичности RNAScope можно считать превосходящим VENTANA PD-L1 и сопоставимым с Dako PD-L1 IHC 22C3 и 28–8.
Но вот смущает следующее, написанное ниже здесь https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6464208/
Threshold optimisation for %TCmRNA and mean dots/tumour cell, results in high specificity to IHC PD-L1 classification, but only moderate sensitivity. The RNAScope assay, if compared to VENTANA PD-L1 (SP263) rabbit mAb assay (as a gold standard), would select a marginally different PD-L1 high patient population using the cut offs derived here.